A knowledge based resource for analysis of Non-ribosomal Peptide Synthetases and Polyketide Synthases



A major component of NRPS-PKS are searchable databases of large multi-enzymatic, multidomain megasynthases involved in biosynthesis of pharmaceutically important natural products like non-ribosomal peptides and polyketides . These databases provide an interface to correlate chemical structures of these natural products with the domains and modules in the corresponding polyketide synthases (PKSs) or non-ribosomal peptide synthetases (NRPSs). The pictorial depictions combined with specific query interfaces facilitate easy identification of various domains and modules from a given polypeptide sequence. NRPS-PKS allows the user to extensively analyze and retrieve the sequence homology of various PKS or NRPS domains providing guidelines for carrying out domain and module swapping experiments. In addition it predicts the specificity of starter and extender precursor units of each potential acyl transferase or adenylation domain.

The frontend of NRPS-PKS is organized as independent units like NRPSDB, PKSDB and ITERDB as per the type of natural product and mechanism of their biosynthesis. However, at the backend they are closely integrated with each other to permit analysis of hybrid NRPS/PKS proteins. NRPS-PKS also provides interface to CHSDB, a database of Type III Polyketide synthases, which are single domain proteins involved in biosynthesis of Chalcone-like products.

ANALYZE HYBRID NRPS/PKS SEQUENCES


NRPSDB :- A database of non-ribosomal peptide synthetases.

PKSDB :- A database of modular polyketide synthases.

ITERDB :- A database of type I Iterative PKS gene clusters.

CHSDB :- A database of Chalcone Synthases.


Comments and suggestions to : Debasisa Mohanty