Mass Spectrometer
The Centralized Mass Spectrometry Facility (CMSF) of NII provides support and services for Protein Identification and Characterization. Core activities includes identification of proteins and determination of post-translational modifications using state of art mass spectrometry. Following are the major equipments at the CMSF:
Liquid Chromatography-Mass Spectrometry (LC-MS/MS) (Make: Thermo Scientific, USA)
A) Mass Spectrometer: Orbitrap Exploris 240
i) UHPLC: Vanquish Neo
ii) MS Data Analysis Software: Proteome Discoverer 3.0 & Mascot Server 2.4
Thermo Orbitrap Exploris 240: The Thermo Orbitrap Exploris 240 HR-MS with Nano-LC has been recently installed in April 2023. As the name suggests, the Exploris 240 system has the resolving power (FWHM) in the range 15,000 to 240,000 at m/z 200 with exceptional mass accuracy. It enables experimental versatility without compromising data certainty. The MS performance has been strongly backed up with Vanquish Neo-UHPLC (Nano-LC) system that has been installed for the very first time in North India. It has the capacity to deliver flow rates in the range 1 nLmin-1 to 100uLmin-1 to exhibit a broad range of high-sensitivity LC-MS workflows.
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Gas Chromatography - Mass Spectrometer (GC-MS): The GC-MS (Agilent 7890A) coupled with 5975C MSD with Triple-Axis Detector (TAD) is a high-performance system. Equipped with advanced separation capabilities and powerful new productivity features; it also has real-time self-monitoring instrument intelligence powered by a 5th-generation electronic pneumatics control (EPC). Additionally, digital electronics for retention time locking (RTL) precision delivers exceptional performance and reliability. Faster oven cool-down and robust backflush capability coupled with advanced automation features and faster GC/MS oven ramps results in enhanced productivity.
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Thermo TSQ Altis Plus: Thermo TSQ Altis Plus is a triple quadrupole mass spectrometer for high confidence targeted compound detection and absolute quantitation in metabolomics studies. TSQ Altis Plus is integrated with Vanquish UHPLC system which maximizes the sample delivery and separation capabilities, maintaining the sensitivity to meet minimum residue limits without costly sample preparation and derivatization steps. The instrument has very high sensitivity and selectivity for low level compound detection and quantitation in complex biological matrices. The system is equipped with Tracefinder 5.1 for data acquisition/processing and analysis.
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